Monday, December 21, 2009

Point of Burden

It comes to end of december as well as end of year 2009.

The burden become more and more as well as stress..



Today i though would be a happy day for me...due to he come back
but end up terrible bad day for me..



Culturing 2 cell lines (Liver cancer cell and Gastric disease carcinoma cell) burden me a lot and bring more stress and tiredness ..
Public holiday and weekend are not rest day for me anymore.
I celebrate my holiday with my cells at lab..
Actually i m quite enjoy doing lab work during public holiday and weekend instead of week day.
The queitness and less people environment let me feel much more relax..



However, public holiday and weekend, there are less transport to and from school..
It brings inconvenient for me..
What to do? I have not my own transport.
That is a point of BURDEN.



Another point of burden is HIM.
I hope i could finish up for culturing 1 cell line (liver cancer cell). But public holiday and festival season now stop me from doing so..
I want to go lab during public holiday and i need him..
but he is always MIA.
What to do?
Struggling for 2 or 3 days..
I dont know what can i do.
I try to do not accept any invitation but it bring danger for me..
Because i need to go and back alone by public transport in so-called danger area..





I HATE!!!!





HepG2 cancer cell line morphology~ (Liver cancer cell)~



*The shining membrane cell is healthy cell.~^^

Thursday, November 26, 2009

Confuse!

Confuse of a day..





I got a new cell line last 2 days. I checked the cell line characteristic through ATCC website and found out it is suspension cell line. Mean that i do no need to use Phosphate Buffer Sulfate (PBS) to wash the cell line and typsin to typsinize the cell when i subculture.





How I know, today my senior told me, although the cell line is suspension but it has anchorage cell line characteristic . (Har..!!)..Because last few sem, FYP senior cultured that kind of cell line after 1 week and found that there is cell attach at the bottom of flask...





It make me confuse..





The new cell line i need to culture is SNU 1.


It is gastric carcinoma. (Why so gam d?? Last time, i suffered from gastic badly...Now in the control state to prevent gastric come to attack..)


More information about SNU 1 refer to http://www.atcc.org/ATCCAdvancedCatalogSearch/ProductDetails/tabid/452/Default.aspx?ATCCNum=CRL-5971&Template=cellBiology





Hope everything will be fine...Pray hard and harder...!!







Miss day of that~~
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This is cheer up day of me...=)

Thursday, October 15, 2009

Say NO!

Panic and worry...


Today I did cell seeding for MTT assay.


1 by 1 incident happen..


It was caused me panic, worry and moodless..


It dont know why my liver cancer cell line is that stubborn.


I trypsinized twice, it was still cant detach.


My conclusion for the cell line is the cell line more confortable to settle down in new flask, so they dont like to detach..(Console myself..)


Faced 1 by 1 incident in the process of cell seeding,I was panic.


Errgh!


No Panic next time please...








*P/s: From beginning of FYP (1st of September), it was my first time i had my lunch at around 2pm..>.<


That time, i was totally surrended.I cant fight with it in order to plate 1 more MTT assay.








Next time PLEASE:


NO-

Panic

Moodless

Geram

Angry

Brain Hang


Yes-

Calm

Plan properly

Believe myself


When face any problem or sudden incident


Give myself



Saturday, September 19, 2009

Cute but Headache

Hep G2...
Something cute but headache for me..
For your information:
Hep G2 is liver cancer cell line.
The disease is hepatocellular carcinoma.
It is anchorage cell line which growth on bottom of T-flask.
It is also like to clump together. (That's problem that cause me headache).
They like to clump together..so they tend to overlap one to each other.
Thus, resuspend take me longer time to separate them into 1 cell to 1 cell condition..
It comes to a matter when my resuspend technique is damn poor...>.<

Hope it hear my pain..Please...

Wednesday, August 19, 2009

Ulu Langat Waterfall

Back to 9th August 2009 (Sunday), somewhere i went: Ulu Langat Waterfall .

Where it is? Somewhere in Selangor lor..


Trip to Ulu Langat Waterfall was held by FES teakwando. Haha, I am not Teakwando member seriously i say. Ulu Langat Waterfall is really a beautiful and nice scene. Play fun alot at there and I miss the Natural 滑梯 so much..>.<. So much natural thing i can do, including NATURAL OSIM, Natural SK II (as RQ said)... Nothing I can describe more. Let's picture talk about everything.

Signboard before heading to Waterfall.



Waterfall


Take rest, before we climb around 4th floor height hill to reach waterfall place.

Reach destination.




Still planing how to wet?!


Natural OSIM. It's not Photoshop effect.



Before back.


^^


Thursday, August 6, 2009

Girls vs Boys.

Just as a random post about different between girls and boys. See carefully. I mean GIRLS and BOYS but NOT MAN. * Haha..isi tersurat here..



Last week, I chatted with my best friend who currently studying Accounting in UUM. That was the first time we had long chat in MSN. The long chat between us need to thank to boys especially our special boyfriend. Yaa. both of us complaint about our bf. *Knowing him will not read my blog, so say something bad about him does not a matter..[evil laugh]:D..

By the way, complain about bf is not a big things ba..?!



I found that both of us have quite same concept towards our own bf. (I think not only both of us ya..it is including most of girls who are falling in love yar..).



  • Her bf tend to join co-curiculum even it is not included in their credit hour since they are year 3 ady. Her bf is member of Askar Wataniah. Thus camping is a thing cant be lacked of and 1 month training before camping is a MUST. Therefore, he need to have hours training everyday. As a result, his result might be influenced. That is what she worry about him and DISLIKE him satisfy his time in co-c rather than study.

Same situation at me here. He like to go to Teakwando training every Tuesday. Last few sem, he went to training every week, in my thought, he was chairman, so that is a responsibility to do so. But now, he resigned his post and still he has a passion go to training every Tuesday, even though some of old committe started to escape from the training. Same worry as my best friend come across. After training and back hime, it is quite late actually plus that time, it is too tired also. So..nothing can be done that time except burn midnight oil..However, tomorrow there is 8 am early MORNING class..


Lesson: Why they like to join co-curiculum so much? For them, co-curiculum can bring them happiness and relax. They also can mix with many friends who have same interest as them. Thus, they can make crazy and joke around.





  • Computer games or online games. Haha, some people when he/she see the title, he/she might know what point that i will point it out..Mostly boys they like to play games. Her bf also like to play computer games but not frequent because he knew his gf dont like him to play games. For example, before exam too stress, play games to release tension..

People who is knowing me, they know i also dont like him to play computer games. Really, at first, i hate him do nothing whole day and just play computer games. I am a quite weird girl that dont really like to play computer game. I do play simple computer games like Suduku, Mahjong Titans, FreeCell, Solitaire and etc. Like Stronghold Legands, Travian, WorldCraft and 三国 , i have not idea how to play and got boring about it then quit it. So, i dont really know why they can face the computer whole day just play it.


Lesson: After i asked him many questions regard the computer game, i found that actually from game, they can learn a lot and release tension. I read from newspaper, play online computer game can improve memory because both of our hand is using to play game so both side brain rather than 1 side brain is also worked at the same time.


So.. Activity and computer games are the boys' favourite that is different with girls. That is not truth for all of the boys and girls. Some girl, they like play game as well; while some boy, they dont like play game. But in my friend and my cases, our bf favourite is not same as us.


So what can we do?


[NO WAY ask either 1 change the habits. Only negotiation and understanding each other can overcome the madness and hatred. ]



Lastly, let me present u L4D. That is L4D (Left 4 Dead) which make those guys highly addicted.


Friday, July 31, 2009

I am emo again.



I am emo again.


and I am confused as well.


Previously, i planned back penang this weekend due to next week, there is no test.


My plan suddenly changed because no one can take me when i reached Juru Tol (As you know, my place dont have a bus-station-like-place, so i need to take bus from pudu to Penang island or Butterworth and stop at Juru Tol.)





My dad and my eldest brothers are not at home. They went outstation... :(








p/s: Everyday thing MUST do is check mail. Waiting my FYP supervisor send me the consultation time in order to meet her..





pp/s: The relationship can cause someone to become more burden and headache. The problem come from both side's parents and the couple's feeling.