I got a new cell line last 2 days. I checked the cell line characteristic through ATCC website and found out it is suspension cell line. Mean that i do no need to use Phosphate Buffer Sulfate (PBS) to wash the cell line and typsin to typsinize the cell when i subculture.
How I know, today my senior told me, although the cell line is suspension but it has anchorage cell line characteristic . (Har..!!)..Because last few sem, FYP senior cultured that kind of cell line after 1 week and found that there is cell attach at the bottom of flask...
It make me confuse..
The new cell line i need to culture is SNU 1.
It is gastric carcinoma. (Why so gam d?? Last time, i suffered from gastic badly...Now in the control state to prevent gastric come to attack..)
More information about SNU 1 refer to http://www.atcc.org/ATCCAdvancedCatalogSearch/ProductDetails/tabid/452/Default.aspx?ATCCNum=CRL-5971&Template=cellBiology
Hope everything will be fine...Pray hard and harder...!!
Miss day of that~~
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This is cheer up day of me...=)
