Today I did cell seeding for MTT assay.
1 by 1 incident happen..
It was caused me panic, worry and moodless..
It dont know why my liver cancer cell line is that stubborn.
I trypsinized twice, it was still cant detach.
My conclusion for the cell line is the cell line more confortable to settle down in new flask, so they dont like to detach..(Console myself..)
Faced 1 by 1 incident in the process of cell seeding,I was panic.
Errgh!
No Panic next time please...
*P/s: From beginning of FYP (1st of September), it was my first time i had my lunch at around 2pm..>.<
That time, i was totally surrended.I cant fight with it in order to plate 1 more MTT assay.
Next time PLEASE:
NO-
Panic
Moodless
Geram
Angry
Brain Hang
Yes-
Calm
Plan properly
Believe myself
When face any problem or sudden incident
Give myself
